{"id":20067,"date":"2023-12-01T11:29:33","date_gmt":"2023-12-01T03:29:33","guid":{"rendered":"https:\/\/web.kcislk.ntpc.edu.tw\/?p=20067"},"modified":"2025-04-23T08:59:17","modified_gmt":"2025-04-23T00:59:17","slug":"20231201-2","status":"publish","type":"post","link":"https:\/\/web.kcislk.ntpc.edu.tw\/en\/20231201-2\/","title":{"rendered":"iGEM\u5b78\u751f\u5be6\u9304\uff08Part II\uff09\uff0d\u300c\u5728\u5df4\u9ece\uff0c\u6211\u770b\u5230\u4e86\u6bcf\u4e00\u500b\u968a\u4f0d\u5c0diGEM \u7684\u71b1\u60c5\u3001\u9032\u6b65\u3001\u548c\u52aa\u529b\uff0c\u9019\u6b21\u7684\u6bd4\u8cfd\u7d93\u9a57\u4e5f\u8b93\u6211\u78ba\u8a8d\u4e86\u6211\u7684\u672a\u4f86\u671f\u671b\u548c\u76ee\u6a19\u3002\u300d"},"content":{"rendered":"
\"\"<\/figure>\n\n\n\n
11B \u738b\u8000\u5fb7<\/h5>\n\n\n\n

\u3000\u3000\u6211\u8eab\u70ba\u4e00\u500b\u95dc\u6ce8\u570b\u969b\u8b70\u984c\u4e14\u4ee5\u751f\u7269\u76f8\u95dc\u79d1\u7cfb\u70ba\u5927\u5b78\u76ee\u6a19\u7684\u5b78\u751f\uff0c\u5728\u5f97\u77e5\u6709iGEM\u9019\u500b\u6bd4\u8cfd\u6642\u611f\u5230\u975e\u5e38\u8208\u596e\u548c\u671f\u5f85\uff0c\u56e0\u70ba\u6211\u5011\u4e0d\u50c5\u53ef\u4ee5\u85c9\u7531\u751f\u7269\u65b9\u9762\u7684\u77e5\u8b58\u548c\u6280\u8853\u53bb\u89e3\u6c7a\u570b\u969b\u8b70\u984c\uff0c\u4e14\u6211\u4e5f\u76f8\u4fe1\u6211\u4e00\u5b9a\u53ef\u4ee5\u5728\u9019\u500b\u6bd4\u8cfd\u4e2d\u5b78\u7fd2\u5230\u66f4\u591a\u7684\u751f\u7269\u65b9\u9762\u77e5\u8b58\u3002<\/p>\n\n\n\n

\u3000\u3000\u5728\u9019\u500bTaipei_KCISLK_v1 \u7684\u5718\u968a\u88e1\uff0c\u8001\u5e2b\u548c\u6211\u5f35\u786f\u60c7\u5b78\u9577\u7684\u7fa4\u7d44\u53eb\u505a\u201cAll Rounders\u201d\u56e0\u70ba\u5728\u5718\u968a\u88e1\u7684\u5404\u9805\u5167\u5bb9\u6211\u5011\u90fd\u6709\u53c3\u8207\uff0c\u4f8b\u5982\uff1aiGEM\u6c7a\u8cfd\u7684\u6524\u4f4d\u8a2d\u8a08\u3001iGEM\u8a55\u5be9\u554f\u7b54\u6642\u7684\u56de\u8986\u3001\u7814\u8a0e\u6703\u7684\u4e3b\u8fa6\u3001\u7d93\u71dfIG\u548c\u8ca0\u8cac\u56de\u8986\u90f5\u4ef6\u7b49\u7b49\u3002\u9664\u4e86\u9019\u4e9b\u201cAll Rounders\u201d\u7684\u5de5\u4f5c\u4e4b\u5916\uff0c\u6211\u81ea\u5df1\u672c\u8eab\u4e5f\u662f\u5be6\u9a57\u7d44\u7684\u6210\u54e1\uff0c\u9019\u4e9b\u5de5\u4f5c\u8b93\u6211\u6536\u7a6b\u826f\u591a\uff0c\u5b78\u5230\u4e86\u975e\u5e38\u591a\u751f\u7269\u4e0a\u7684\u5b78\u8853\u77e5\u8b58\uff0c\u9084\u6709\u4eba\u969b\u4ea4\u6d41\u7684\u7d93\u9a57\u3002<\/p>\n\n\n\n

\u3000\u3000\u5176\u5be6\u525b\u958b\u59cb\u7d44\u968a\u6642\uff0c\u6211\u4e5f\u6703\u61f7\u7591\u81ea\u5df1\u662f\u5426\u6709\u8db3\u5920\u7684\u80fd\u529b\u53bb\u53c3\u8207\u9019\u500b\u6bd4\u8cfd\uff0c\u4f46\u6700\u5f8c\u9084\u662f\u6210\u529f\u901a\u904e\u4e86\u9762\u8a66\u52a0\u5165Taipei_KCISLK_v1 \u9019\u500b\u5927\u5718\u968a\u3002\u5728\u525b\u958b\u59cb\u7684\u751f\u7269\u5b78\u8853\u77e5\u8b58\u8ab2\u7a0b\u4e2d\uff0c\u81ea\u5df1\u662f\u6709\u4e00\u9ede\u8ddf\u4e0d\u4e0a\u7684\uff0c\u53ea\u8981\u9047\u5230\u751f\u7269\u76f8\u95dc\u7684\u554f\u984c\uff0c\u6211\u90fd\u6703\u81ea\u5df1\u5148\u60f3\u8fa6\u6cd5\u67e5\u8a62\u8cc7\u6599\u518d\u53bb\u548c\u8001\u5e2b\u8a0e\u8ad6\uff0c\u9019\u6a23\u6211\u624d\u80fd\u78ba\u5be6\u7684\u7406\u89e3\u5167\u5bb9\uff0c\u7d93\u904e\u8a8d\u771f\u7684\u4e0a\u8ab2\uff0c\u4ee5\u53ca\u5411\u8001\u5e2b\u8acb\u6559\u5f8c\uff0c\u6211\u624d\u6162\u6162\u6210\u529f\u638c\u63e1\u5be6\u9a57\u6b65\u9a5f\u548c\u751f\u7269\u7406\u8ad6\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u3000\u3000\u8eab\u70ba\u53f0\u70632023\u5e74iGEM\u7814\u8a0e\u6703\u7684\u4e3b\u8fa6\u4eba\u4e4b\u4e00\uff0c\u5f9e8\u6708\u7684\u7814\u8a0e\u6703\u5230\u6cd5\u570b\u7684\u6c7a\u8cfd\uff0c\u6211\u770b\u5230\u4e86\u4e0d\u53ea\u6797\u53e3\u5eb7\u6a4b\u7684\u540c\u5b78\u7684\u6210\u9577\uff0c\u800c\u662f\u5168\u53f0\u7063\u9ad8\u4e2d\u548c\u5927\u5b78\u4ee3\u8868\u968a\u7684\u6210\u9577\u3002\u8a31\u591a\u9ad8\u4e2d\u968a\u4f0d\u5728\u7814\u8a0e\u6703\u6642\uff0c\u5c0d\u81ea\u5df1\u7d44\u5225\u7684\u5167\u5bb9\u4e26\u4e0d\u662f\u5f88\u719f\u6089\uff0c\u5305\u542b\u6211\u5011\u6797\u53e3\u5eb7\u6a4b\u3002\u4f46\u7d93\u904e\u5169\u500b\u591a\u6708\u7684\u9b54\u9b3c\u8a13\u7df4\u5f8c\uff0c\u5728\u5df4\u9ece\u6c7a\u8cfd\u7684\u821e\u53f0\uff0c\u6211\u770b\u5230\u4e86\u6bcf\u4e00\u968a\u53f0\u7063\u9ad8\u4e2d\u751f\u5c0diGEM \u7684\u71b1\u60c5\u3001\u9032\u6b65\u3001\u548c\u52aa\u529b\u3002\u5728\u7814\u8a0e\u6703\u6709\u975e\u5e38\u591a\u5c08\u696d\u4eba\u58eb\u63d0\u4f9b\u4e86\u5404\u968a\u975e\u5e38\u591a\u5efa\u8b70\u548c\u53cd\u994b\uff0c\u4f8b\u5982\uff1a\u9ad8\u7aef\u526f\u7e3d-\u9023\u52a0\u6069\u91ab\u5e2b\u3001\u53f0\u5927\u751f\u6280\u7cfb\u6797\u6649\u7384\u6559\u6388\u7b49\u7b49\u3002\u6211\u5e0c\u671b\u660e\u5e74\u9084\u80fd\u7e7c\u7e8c\u5ef6\u7e8c\u627f\u8fa6\u53f0\u7063\u7684iGEM\u7814\u8a0e\u6703\uff0c\u805a\u96c6\u53f0\u7063\u5404\u5730\u7684\u9ad8\u4e2d\u548c\u5927\u5b78\u5c0d\u5408\u6210\u751f\u7269\u5b78\u611f\u8208\u8da3\u7684\u540c\u5b78\u5011\u805a\u96c6\u5728\u4e00\u8d77\uff0c\u4e92\u76f8\u7d66\u4e88\u53cd\u994b\u548c\u8a0e\u8ad6\u3002<\/p>\n\n\n\n

\u3000\u3000\u6211\u5e0c\u671b\u6211\u5011\u7684\u5be6\u9a57\u53ef\u4ee5\u7e7c\u7e8c\u88ab\u5ef6\u7e8c\u505a\u4e0b\u53bb\uff0c\u76ee\u524d\u6211\u5011Taipei_KCISLK_v1 \u7684\u6210\u679c\u9084\u8655\u65bc\u7406\u8ad6\u968e\u6bb5\uff0c\u6211\u5011\u7684\u5be6\u9a57\u5df2\u7d93\u8b49\u660e\u4e86\u6211\u5011\u6539\u9020\u904e\u7684\u5927\u8178\u687f\u83cc E coli. \u76ca\u751f\u7b98\u5e73\u53f0\u662f\u53ef\u4ee5\u6210\u529f\u900f\u904eOleate Indiction\u53bb\u91cb\u653e\u6211\u5011\u8981\u7684\u8693\u6fc0\u9176\u86cb\u767d\u4f86\u89e3\u6c7a\u4eba\u9ad4\u88e1\u9762\u7684\u8840\u6813\u554f\u984c\uff0c\u5e0c\u671b\u6211\u5011\u660e\u5e74\u7684Taipei_KCISLK_v2\u53ef\u4ee5\u6210\u529f\u5c07\u6211\u5011\u7684\u5be6\u9a57\u7e7c\u7e8c\u505a\u4e0b\u53bb\uff0c\u5305\u542b\u63db\u7b98\uff0c\u5c07\u76ee\u524d\u7684E coli. BL21\u63db\u5230E coli. nissle 1917\u624d\u80fd\u5728\u4eba\u9ad4\u751f\u5b58\uff0c\u6216\u751a\u81f3\u662f\u63db\u5230\u5e03\u62c9\u9175\u6bcd\u83cc\u4f5c\u70ba\u76ca\u751f\u7b98\u5e73\u53f0\u7684\u4e3b\u9ad4\u7b49\uff0c\u8b93\u6211\u5011\u7684\u7814\u7a76\u53ef\u4ee5\u771f\u7684\u904b\u7528\u5728\u4eba\u9ad4\u4e0a\uff0c\u89e3\u6c7a\u570b\u969b\u8b70\u984c\uff0d\u8840\u6813\u3002<\/p>\n\n\n\n

\u3000\u3000\u6211\u81ea\u5df1\u500b\u4eba\u975e\u5e38\u559c\u6b61\u751f\u7269\uff0c\u5c24\u5176\u662f\u8207\u4eba\u9ad4\u76f8\u95dc\u7684\u90e8\u5206\uff0c\u900f\u904e\u9019\u6b21\u7684\u5be6\u9a57\u548c\u6bd4\u8cfd\u7d93\u9a57\uff0c\u8b93\u6211\u78ba\u8a8d\u4e86\u6211\u81ea\u5df1\u7684\u672a\u4f86\u671f\u671b\u548c\u76ee\u6a19\u3002\u5e0c\u671b\u4ee5\u5f8c\u53ef\u4ee5\u6301\u7e8c\u505a\u76f8\u95dc\u7814\u7a76\uff0c\u7e7c\u7e8c\u6df1\u6839\u5728\u751f\u7269\u9019\u500b\u9818\u57df\u4e2d\uff0c\u540c\u6642\u8b93\u66f4\u591a\u4eba\u4e86\u89e3\u9019\u500b\u793e\u6703\u4f9d\u8cf4\u6027\u975e\u5e38\u9ad8\u7684\u79d1\u7cfb\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n
12A\u5f35\u786f\u60c7<\/h5>\n\n\n\n

\u3000\u3000\u7576\u521d\u807d\u5b8c\u8aaa\u660e\u6703\u5f8c\uff0c\u89ba\u5f97\u9019\u500b\u6bd4\u8cfd\u5f88\u6709\u8da3\u65bc\u662f\u5c31\u6c7a\u5b9a\u53c3\u52a0\u4e86\u3002\u96d6\u7136\u6211\u540d\u7fa9\u4e0a\u662f\u5be6\u9a57\u7d44\u7684\u6210\u54e1\uff0c\u4f46\u662f\u6211\u8ca0\u8cac\u7684\u4e0d\u50c5\u9650\u65bc\u5be6\u9a57\uff0c\u50cf\u662f\u5168\u53f0\u7814\u8a0e\u6703\u3001\u8a2a\u8ac7\u3001\u53cd\u8f49\u6559\u5b78\uff0c\u4ee5\u53ca\u5be6\u9a57\u7b46\u8a18\u7d71\u6574\u7b49\u6211\u90fd\u6709\u53c3\u8207\u3002\u56e0\u70ba\u5718\u968a\u4eba\u54e1\u6bd4\u8f03\u5c11\u7684\u95dc\u4fc2\uff0c\u6211\u5011\u7121\u6cd5\u505a\u5230\u5f88\u5c08\u4e00\u7684\u5206\u5de5\uff0c\u6240\u4ee5\u76f8\u5c0d\u4f86\u8aaa\uff0c\u6211\u5011\u5718\u968a\u7684\u6bcf\u500b\u4eba\u90fd\u8981\u5177\u5099\u8de8\u9818\u57df\u80fd\u529b\uff0c\u4e26\u4e14\u6e05\u695a\u77e5\u9053\u6574\u500b\u7814\u7a76(\u5f9e\u5be6\u9a57\u5ba4\u88e1\u5230\u5be6\u9a57\u5ba4\u5916)\u5230\u5e95\u5728\u505a\u4ec0\u9ebc\u3002<\/p>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u3000\u3000\u5373\u4f7f\u904e\u7a0b\u4e26\u4e0d\u8f15\u9b06\uff0c\u6211\u9084\u662f\u8a8d\u70ba\u80fd\u8ddf\u5c0d\u65bc\u9019\u500b\u9818\u57df\u6709\u8208\u8da3\u7684\u5176\u4ed6\u9ad8\u4e2d\u751f\u53ca\u5927\u5b78\u751f\u4ea4\u6d41\u5341\u5206\u5f88\u958b\u5fc3\uff0c\u4e5f\u4ea4\u5230\u4e86\u5f88\u591a\u7684\u597d\u670b\u53cb\u3002\u5230\u5df4\u9ece\u5f8c\uff0c\u6211\u89ba\u5f97\u6211\u505a\u7684\u9019\u4e00\u5207\u90fd\u503c\u5f97\u4e86\uff0c\u771f\u7684\u8eab\u8655\u73fe\u5834\uff0c\u5c31\u6703\u767c\u89ba\u539f\u4f86\u6709\u9019\u9ebc\u591a\u4eba\u8ddf\u6211\u5011\u4e00\u6a23\u60f3\u8981\u6539\u8b8a\u4e16\u754c\uff0c\u9019\u4e5f\u8b93\u6211\u4e0b\u5b9a\u6c7a\u5fc3\u672a\u4f86\u60f3\u8981\u5f80\u9019\u65b9\u9762\u7e7c\u7e8c\u5b78\u7fd2\u3002<\/p>\n\n\n\n

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